Method Validation for Analysis of Simvastatin in Human Plasma Using Liquid Chromatography Tandem Mass Spectrometry (LC-MS-MS)
Published: December 1, 2013 | DOI: https://doi.org/10.7860/JCDR/2013/.3748
Khaled M. Alakhali
1. (a) School of Pharmacy, Universiti Sains Malaysia.
(b) College of Pharmacy, King Khalid University, Abha, Kingdom of Saudi Arabia.
(c) Department of Pharmacy, Medical school in Thamar University, Republic of Yemen.
Correspondence
Dr. khaled M. Alakhali,
Post box no:1882, Postal code: 61441, Abha, Kingdom of Saudi Arabia.
Phone: 00966558649740, E-mail: Khaled_akhali@yahoo.com
Introduction:The Liquid Chromatography Tandem Mass Spectrometry (LC-MS-MS) for determination simvastatin in human plasma has been developed after extraction by by ethyl acetate and hexane (90/10%, v/v) using lovastatin as internal standard.
Material and Methods:The mobile phase consisting of mixture of acetonitrile and water (75/25%, v/v) 500µL/min by separated the solutes on a C18 column.
Discussion:The lower limit of quantitation of 0.25 ng/mL was achieved when the calibration curve was linear from 0.25-50 ng/mL. The entire run time for analysis was only 6 min. The quantitation in the selective reaction monitoring (SRM) in positive ion mode, the daughter ions m/z 325 for simvastatin and m/z 285 for lovastatin were used. The Parent ions in positive ion mode were m/z 441.3 for simvastatin and m/z 405.1 for lovastatin. The intra-day coefficients of variation were less than 14% while the inter-day coefficients of variation were less than 10%.
Conclusion:The LC-MS-MS detection is sensitive due to its capability to eliminate interferences from endogenous components.
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